This chapter discusses poly ADP-ribosylation of protein. 14, POLY ADP-RIBOSYLATION OF PROTEIN POLY(ADP-RIBOSE) SYNTHETASE 15 No additions V max. Protein de-poly-ADP ribosylation tagged: biochemistry, protein modifications, nad, nicotinamide, dna polymerase, parg. Protein de-poly-ADP ribosylation.
Endogenous protein mono- ADP- ribosylation in Arabidopsis thaliana. Protein mono- ADP- ribosylation post- translationally transfers the ADP- ribose moiety from the . This type of modification has been widely characterized and shown to regulate protein activities in animals, yeast and prokaryotes, but has never been reported in plants. In this study, using . One protein substrate of 3. Heat treatment, protease sensitivity and nucleotide derivative competition assays suggested a covalent reaction of NAD+ with the 3. Poly (ADP- ribose) polymerase inhibitor failed to suppress the reaction, but chemicals that destroy mono- ADP- ribosylation on specific amino acid residues could break up the linkage, suggesting that the reaction was not a poly- ADP- ribosylation but rather a mono- ADP- ribosylation. This modification mainly existed in leaves and was enhanced by oxidative stresses. In young seedlings, two more protein substrates with the size of 4. Although the substrate proteins remain to be identified, this is the first report on the characterization of endogenously mono- ADP- ribosylated proteins in plants. Keywords. Arabidopsis thaliana.
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